NEXTFLEX® Rapid DNA-Seq Kit 2.0
Price on request
Delivery time: 2 to 3 weeks
Product number:
NOVA-5188-01
Product information "NEXTFLEX® Rapid DNA-Seq Kit 2.0"
Cell free DNA has become a powerful marker in clinical research applications due to the unique origin of DNA molecules present in plasma or serum. Detection of fetal DNA from maternal plasma has proven to be a viable, non-invasive option to identify a variety of fetal traits including: sex determination, sex chromosome-linked disorders and aneuploidy events. Circulating tumor DNA extracted from plasma of symptomatic patients can be used as a non-invasive resource for research towards diagnosis, prognosis, treatment decisions, and follow-up monitoring of cancer patients. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 is designed for 3 hour DNA library construction of cell free fetal or circulating tumor DNA. The kit can be used to prepare single, paired-end and multiplexed DNA libraries for sequencing using Illumina® platforms. NEXTFLEX® 1-step End-Repair and Adenylation protocol simplifies workflow and shortens hands-on library construction time. In addition, the availability of up to 384 unique adapter barcodes facilitates high-throughput applications. There are three main steps involved in preparing cell free DNA for sequencing: DNA End Repair & Adenylation, Adapter Ligation and PCR Amplification. The optional Gel-Free Nucleosome Enchriment step, performed before End Repair & Adenylation, is designed to enrich for mononucloesomes or mono-, di-, and tri-nucleosomes. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 contains the necessary material, except barcodes, to take the user’s purified cell free DNA through library preparation and amplification for loading onto Illumina flow cells for sequencing. The NEXTFLEX® Cell Free DNA-Seq Kit 2.0 is intended for research use only.
| Product type: | Library preparation |
|---|---|
| Regulatory status: | RUO |
| Platform: | Element Biosciences®, Illumina® |
| Storage temperature: | 2 to 8 °C and -20 °C |
| Shipping method: | Room temperature and dry ice |
| Country of distribution: | Germany |