NEXTFLEX® RiboNaut™ rRNA Depletion Kit
Price on request
Delivery time: 2 to 3 weeks
Product number:
NOVA-512961
Product information "NEXTFLEX® RiboNaut™ rRNA Depletion Kit"
The NEXTFLEX® RiboNaut™ rRNA Depletion Kit (Human / Mouse / Rat) is designed to be compatible with human, mouse, and rat total RNA. The method used in the NEXTFLEX® RiboNaut™ rRNA Depletion Kit is based on subtractive hybridization, using a mixture of biotinylated oligonucleotides complementary to rRNAs. After hybridization of the bait oligos to total RNA, the rRNA/bait complex and excess bait oligos are removed by capture onto streptavidin-coated magnetic beads. The remaining RNA, which has been depleted of both cytoplasmic and mitochondrial rRNAs, is then purified and concentrated using magnetic beads. Input amounts of total RNA ranging from 5 ng to 1 µg can be processed. The kit has been designed for use with human RNA; however, due to the nature of the probes, depletion of rRNA from mouse and potentially other mammalian species is also possible using this kit. RNA extracted from formalin fixed paraffin embedded (FFPE) samples may also be used. RNA-seq libraries made with rRNA-depleted intact RNA processed using this kit typically comprise less than 1% of the reads obtained in RNA-seq experiments. rRNA reads from highly degraded or FFPE total RNA samples may be higher. We recommended pairing the NEXTFLEX®
RiboNaut™ rRNA Depletion Kit (H/M/R) with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.
| Product type: | Nucleic acid purification |
|---|---|
| Regulatory status: | RUO |
| Storage temperature: | 2 to 8 °C and -20 °C |
| Shipping method: | Room temperature and dry ice |
| Country of distribution: | Germany |