NEXTFLEX® Universal Blockers
Price on request
Delivery time: 2 to 3 weeks
Product number:
NOVA-5143231
Product information "NEXTFLEX® Universal Blockers"
The NEXTFLEX® Universal Blockers are designed to prevent concatemerization of library molecules during target capture hybridizations via the adapter sequences that are added to all library molecules before enrichment. Each strand of these adapter sequences can hybridize with the complementary adapter sequence strand of another library molecule during this enrichment hybridization, creating a "daisy-chain" effect between off-target library molecules and the on-target library molecules that are the intended targets of the capture probe set used in the enrichment hybridization. By blocking this complementary adapter sequence interaction, the number of on-target reads improves dramatically and the depth of enrichment increases. The NEXTFLEX® Universal Blockers are designed for use with Illumina® indexed adapters. They are effective in blocking single-indexed or dual-indexed libraries, without being affected by index length or the presence of a unique molecular index (UMI). The NEXTFLEX® Universal Blockers are designed to target both strands of the adapter sequence regions. The NEXTFLEX® Universal Blockers are compatible with ligation-based workflows, such as the libraries prepared with NEXTFLEX® DNA Barcodes, NEXTFLEX-96™ DNA Barcodes, NEXTFLEX HT™ Barcodes, NEXTFLEX® Dual-Index Barcodes and NEXTFLEX® Unique Dual Index Barcodes. They are also compatible with tagmentation-based workflows, such as Illumina® Nextera™ library prep kits.
| Product type: | Library preparation |
|---|---|
| Regulatory status: | RUO |
| Platform: | Illumina® |
| Storage temperature: | -20 °C |
| Shipping method: | Dry ice |
| Country of distribution: | Germany |